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Purigen Launches New Extraction and Purification Kit that Enables Scientists to Maximize Usable DNA from Limited Biological Samples

Automated Ionic® Cells to Pure DNA Low Input Kit consistently recovers up to twice the amount of high-quality nucleic acid from as few as 10 cultured or sorted cells

Purigen Biosystems, Inc., a leading provider of next-generation technologies for extracting and purifying nucleic acids from biological samples, today announced the launch of the Ionic® Cells to Pure DNA Low Input Kit for researchers working with limited biological samples. The simplified and automated 60-minute workflow delivers high-quality DNA for the rapid investigation of genetic abnormalities or examination of disease treatment effects.

This press release features multimedia. View the full release here: https://www.businesswire.com/news/home/20201013005344/en/

The Ionic Cells to Pure DNA Low Input Kit from Purigen Biosystems (Photo: Business Wire)

The Ionic Cells to Pure DNA Low Input Kit offers consistent recovery of DNA with yields near the theoretical maximum for as many as 100,000 down to as few as 10 cultured or sorted cells. Compared to leading column-based products, the new kit delivers up to twice the amount of DNA with a significantly higher proportion greater than 20 kb in length. Regardless of the input amount, the workflow is the same and does not require carrier RNA. The prepared DNA is ready for analysis by downstream techniques such as next-generation sequencing (NGS) or qPCR.

The Ionic® Cells to Pure DNA Low Input Kit uses Purigen’s innovative isotachophoresis-based (ITP) technology, which is incorporated into its Ionic® Purification System, a compact benchtop instrument that enables the automated extraction of pure and abundant nucleic acids from a wide range of sample types. Biological samples are gently lysed and then loaded into the Ionic® Fluidic Chip. The Ionic System applies an electric field to the chip, and nucleic acids are isolated in their natural, native form using ITP technology. The nucleic acids are not denatured or dehydrated, nor are they bound and stripped from fixed surfaces. The process minimizes fragmentation and eliminates any bead or buffer contamination.

“Unlocking the genetic information stored within precious and limited samples has been a longstanding challenge for genomic researchers,” said Barney Saunders, PhD, CEO of Purigen Biosystems. “Unlike conventional technologies, the Ionic Purification System’s performance does not decline with limited sample input. The Ionic Cells to Pure DNA Low Input Kit simplifies the extraction process, accelerates turnaround times, and improves yields of pure, abundant, and ready-to-sequence DNA from limited sample quantities.”

The Ionic® Cells to Pure DNA Low Input Kit consists of six chips and a reagent set with all necessary enzymes and buffers to perform DNA extraction from 48 samples. The kit is available now through Purigen’s direct sales channels. For more information, please visit: https://www.purigenbio.com/applications/ionic-cells-pure-dna.

About Purigen Biosystems

Purigen Biosystems is redefining nucleic acid sample preparation with an innovative platform based on the highly efficient isotachophoresis technology invented by Juan Santiago, PhD, and his team at Stanford University. Purigen’s automated benchtop instrumentation and accompanying microfluidic chip purify nucleic acid samples from a wide variety of sources, including minute or otherwise challenging

Troilus Discovers New Goldfield Boulder Zone 36 Km From Troilus Mine With Samples up to 26.2 g/t Gold and 27.8 g/t Silver

Figure 1

Troilus Property, Regional Geology and Location of the New Goldfield Zone
Troilus Property, Regional Geology and Location of the New Goldfield Zone
Troilus Property, Regional Geology and Location of the New Goldfield Zone

Figure 2

Close-up of the Goldfield Boulder Zone
Close-up of the Goldfield Boulder Zone
Close-up of the Goldfield Boulder Zone

TORONTO, Oct. 08, 2020 (GLOBE NEWSWIRE) — Troilus Gold Corp. (TSX: TLG) (OTCQB: CHXMF) (“Troilus” or the “Company”) is pleased to announce the latest results from its summer 2020 regional exploration program on its 100%-owned Troilus Project, located within the Frôtet-Evans Greenstone Belt of northern Quebec. The program focused on identifying high priority targets within the extensive land package Troilus acquired and staked earlier in the year (the “Troilus-Frôtet Property”) (see press releases dated April 28, 2020 and July 21, 2020). Initial results collected 28 km southwest of the recently announced Beyan Gold Zone Discovery (see press release dated September 30, 2020), have outlined another new zone of mineralization: the Goldfield Boulder Zone (“Goldfield”). Gold bearing boulders and outcrop can be traced and defined in situ over a minimum strike length of 4 km. Goldfield is accessible from the Route du Nord highway.

Highlights from the New Goldfield Zone:

  • Grab samples returned up to 26.2 g/t gold and 27.8 g/t silver in outcrop

  • Other sample highlights include: 6.51 g/t Au, 5.96 g/t Au, 4.31 g/t Au; 14.0 g/t Ag, 8.6 g/t Ag (see full results in Table 1)

  • Many geological characteristics linking Goldfield to the main mineralized zones over 36 km away

“The discovery of the Goldfield Zone this summer marks an exciting moment for Troilus,” said Justin Reid, CEO of Troilus. “Earlier this year we had the opportunity to significantly increase our land holdings through a combination of acquisition and staking. The addition of the Troilus-Frôtet Property increased our land holdings from 16,000 hectares to over 107,000 hectares. Our evolving geologic model, developed over the last couple of years through drilling and exploration around the main ore bodies of Z87, the J Zones and more recently the Southwest Zone, led us to believe that the region could have district scale potential and we believed that expanding our claim area had the potential to add significant value for shareholders via discovery. The Goldfield Zone, located on the western side of the Troilus-Frôtet Property, marks the furthest afield exploration conducted to date. We are thrilled to report newly discovered, near-surface gold occurrences with samples ranging up to 26.2 g/t gold and 27.8 g/t silver, 36 km from the main mineralized zones at the Troilus property. The Goldfield Zone results, combined with the recent Beyan Zone results, suggest that gold mineralization within the Frôtet-Evans Greenstone Belt is prolific and not constrained geographically as historically thought. We await further results from locations across the Troilus-Frotêt Property collected during the summer and believe that these showings could just be the beginning of what the district could host.”

During this initial bedrock mapping and boulder tracing, the Troilus Geological team located several mineralized boulders on the main Tortigny block assaying up to 26 g/t Au